Ng Wing Keong Prof


Universiti Sains Malaysia
M.Sc., Asian Institute of Technology, Thailand
Ph.D., University of California, Davis, USA
Post-doctoral, Mississippi State University, USA
Ext. 4005;
Aquaculture Nutrition


Research Profile

Areas of research interest and specialization includes aquatic animal nutrition, feed technology, palm oil use in aquafeeds, microbial biotechnology, aquaculture systems, ornamental fish, shrimp culture, fish physiology and coastal ecosystems. Special focus is given towards research projects that are aimed at developing a better understanding of nutrients utilization by fish and shrimp. This includes the evaluation of novel protein and lipid sources in aquafeeds to improve the economic and environmental sustainability of aquaculture operations. Previous and current research projects have involved working with various tropical and temperate aquatic organisms such as tilapia, catfishes, carps, groupers, black sea bream, salmonids, sturgeon, ornamental fish, freshwater prawns, marine shrimp and seaweeds.

Current Research


  • Alternative protein and lipid sources in aquaculture feeds with special focus on palm oil-based products and by-products.
  • Bioavailability and biopotency of vitamin E (tocopherols and tocotrienols) in fish and shrimp feeds.
  • The use of dietary organic acids as an alternative to antibiotic growth promoters and for disease control in intensive aquaculture systems.
  • The role and effects of dietary lipids, vitamin E and carotenoids on the reproductive performance of fish and prawns.
  • The nutrient requirements of the Malaysian mahseer (kelah), Tor tambroides.
  • Nutrigenomics in the giant freshwater prawn, Macrobrachium rosenbergii.
  • The replacement of trash fish with cost-effective and nutrient-optimized pelleted feeds for tropical marine fish.
  • The utilization of various palm oil fractions in the diets of coldwater fish such as Atlantic salmon and rainbow trout.
  • Effects of diet on fish fillet quality and sensory characteristics in relation to human health, with special focus on the role of omega-3 long-chain polyunsaturated fatty acids.
  • Tilapia aquaculture in Asia with emphasis on production strategies and feed management.
  • Seaweed extracts as growth enhancers for microalgae culture for use in shrimp hatcheries.
  • The protein and amino acid, lipid and fatty acids, energy, vitamin and mineral requirements of commercially Important farmed food fish and shellfish.
  • The use of feed additives such as feed enzymes to enhance nutrient utilization in fish fed plant protein sources.


  • Food and Agriculture Organization (FAO) of the United Nations.
  • Network of Aquaculture Centres in Asia-Pacific (NACA).
  • Institute of Aquaculture, University of Stirling, United Kingdom.
  • Commonwealth Scientific and Research Organization (CSIRO), Australia.
  • University of Tasmania, Australia.
  • Deakin University, Australia.
  • University of Guelph, Canada.
  • Zhejiang University, P.R. China.
  • Pukyong National University, South Korea.
  • WorldFish Center.
  • Malaysian Palm Oil Board (MPOB), Malaysia.
  • Universiti Malaysia Sabah, Malaysia.
  • Universiti Malaya, Malaysia.
  • National Fish Health Centre, Department of Fisheries, Malaysia.
  • Universiti Putra Malaysia, Malaysia.
  • Department of Agriculture, Sarawak, Malaysia.
  • Malaysian Agricultural and Research Development Institute, Malaysia.
  • Various local and international feed-related companies and organizations.

Selected Publications

  • Ng W.K., Koh C.B. (2016). The utilization and mode of action of organic acids in the feeds of cultured aquatic animals. Reviews in Aquaculture (in press) doi: 10.1111/raq.12141.
  • Koh C.B., Romano N., Siti-Zahrah A., Ng W.K. (2016). Effects of a dietary organic acids blend and oxytetracycline on the growth, nutrient utilization and total cultivable gut microbiota of the red hybrid tilapia, Oreochromis sp., and resistance to Streptococcus agalactiae. Aquaculture Research 47: 357-369.
  • Ng W.K., Koh C.B., Teoh C.Y., Romano N. (2015). Farm-raised tiger shrimp, Penaeus monodon, fed commercial feeds with added organic acids showed enhanced nutrient utilization, immune response and resistance to Vibrio harveyi challenge. Aquaculture 449: 69-77.
  • Rohani-Ghadikolaei K., Abdolalian E., Hojatollah F., Masoud G. & Ng W.K. (2015). The nutritional effect of Isochrysis galbana and Chaetoceros muelleri cultured with different seaweed extracts on the larval development, growth and survival of the marine shrimp, Penaeus indicus. Aquaculture Research 46: 1444-1454.
  • Romano N., Koh C.B., Ng W.K. (2015). Dietary microencapsulated organic acids blend enhances growth, phosphorus utilization, immune response, hepatopancreatic integrity and resistance against Vibrio harveyi in white shrimp, Litopenaeus vannamei. Aquaculture 435: 228-236.
  • Shapawi R., Ebi I. & Ng W.K. (2014). Optimizing the growth performance of brown-marbled grouper, Epinephelus fuscoguttatus (Forskal), by varying the proportion of dietary protein and lipid levels. Animal Feed Science and Technology 191: 98-105.
  • Ng W.K., Kim Y.C., Romano N., Koh C.B. & Yang S.Y. (2014). Effects of dietary probiotics on the growth and feeding efficiency of red hybrid tilapia, Oreochromis sp., and subsequent resistance to Streptococcus agalactiae. Journal of Applied Aquaculture 26: 22-31.
  • Teoh C.Y. & Ng W.K. (2013). Evaluation on the impact of dietary petroselinic acid on the growth performance, fatty acid composition and efficacy of long chain-polyunsaturated fatty acids biosynthesis of farmed Nile tilapia. Journal of Agricultural and Food Chemistry 61: 6056-6068.
  • Lee K.S., Yuen K.H. & Ng W.K. (2013). Deposition of tocopherol and tocotrienol in the tissues of red hybrid tilapia, Oreochromis sp., fed vitamin E free diets supplemented with different plant oils. Fish Physiology and Biochemistry 39:1457-1471.
  • Ng W.K. & Romano N. (2013). A review of the nutrition and feeding management of farmed tilapia throughout the culture cycle. Reviews in Aquaculture 5: 220-254 (invited review).
  • Turchini G.M., Ng W.K. & Tocher D.R., Editors (2010). Fish Oil Replacement and Alternative Lipid Sources in Aquaculture Feeds. CRC Press, Taylor & Francis Group, USA (ISBN 978-1-4398-0862-7).
  • Ng W.K., Koh C.B., Sudesh K. & Siti-Zahrah A. (2009). Effects of dietary organic acids on growth, nutrient digestibility and gut microflora of red hybrid tilapia, Oreochromis sp., and subsequent survival during a challenge test with Streptococcus agalactiae. Aquaculture Research 40: 1490-1500.
  • Turchini G.M., Torstensen B.E. & Ng W.K. (2009). Fish oil replacement in finfish nutrition. Reviews in Aquaculture 1: 10-57. (Invited review).
  • Ng W.K. & Bahurmiz O.M. (2009). The impact of dietary oil source and frozen storage on the physical, chemical and sensorial quality of fillets from market-size red hybrid tilapia, Oreochromis sp. Food Chemistry 113: 1041-1048.
  • Ng W.K. (2009). The current status and future prospects for the aquaculture industry in Malaysia. World Aquaculture 40: 26-30.
  • Ng W.K., Thampi Samraj Y.C., Muthuraman A.L., Editors (2008). Aquaculture Nutrition and Feeds Research in India. Rajiv Gandhi Centre for Aquaculture, Ministry of Commerce & Industry, Government of India, 116 pp. (ISBN 978-81-907390-30).
  • Ng W.K., Abdullah N. & DeSilva S.S. (2008). The dietary protein requirement of the Malaysian mahseer, Tor tambroides (Bleeker), and the lack of protein sparing action by dietary lipid. Aquaculture 284: 201-206.
  • Ng W.K., Wang Y. & Yuen K.H. (2008). Palm vitamin E for aquaculture feeds. Journal of Oil Palm Research, Special Issue: 1-7. (Invited article).
  • Shapawi R., Mustafa S. & Ng W.K. (2008). Effects of dietary fish oil replacement with vegetable oils on growth and tissue fatty acid composition of humpback grouper, Cromileptes altivelis (Valenciennes). Aquaculture Research 39: 315-323.
  • Shapawi R., Ng W.K. & Mustafa S. (2007). Replacement of fish meal with poultry-by product meal in diets formulated for the humpback grouper, Cromileptes altivelis. Aquaculture 273: 118-126.
  • Ng W.K. & Hanim R. (2007). Performance of genetically improved Nile tilapia compared to red hybrid tilapia fed diets containing two protein levels. Aquaculture Research 38: 965-972.
  • Ng W.K., Tocher D.R. & Bell J.G. (2007). The use of palm oil in aquaculture feeds for salmonid species. European Journal of Lipid Science and Technology 109: 394-399. (Invited review article).
  • Bahurmiz O.M. & Ng W.K. (2007). Effects of dietary palm oil source on growth, tissue fatty acid composition and nutrient digestibility of red hybrid tilapia, Oreochromis sp., raised from stocking to marketable size. Aquaculture 262: 382-392.
  • Ng W.K., Minyt Soe & Hla Phone (2007). Aquafeeds in Myanmar: A change from farm-made to factory-made feeds. Aquaculture Asia 12: 7-12.
  • Ng W.K. & Ng C.K., Editors (2006). Asian Aquafeeds: Current Developments in the Aquaculture Feed Industry. Malaysian Fisheries Society Occasional Publication No. 13, 224 pp. (ISBN 983-99563-8-8).
  • Ng C.K., Christianus A. & Ng W.K., Editors (2006). Recent Developments and Technologies in the Aquarium Industry. Malaysian Fisheries Society Occasional Publication No. 12, 256 pp. (ISBN 983-99563-7-X).
  • Ng W.K., Koh C.B. & Zubir B.D. (2006). Palm oil-laden spent bleaching clay as a substitute for marine fish oil in the diets of Nile tilapia, Oreochromis niloticus. Aquaculture Nutrition 12: 459-468.
  • Mohammed-Suhaimee A.M., Zainoha Z. & Ng W.K. (2006). The inclusion of urea-treated palm kernel meal as a feed ingredient in the diet of juvenile seabass (Lates calcarifer) affects growth performance. Journal of Aquaculture in the Tropics 21: 51-68.
  • Ng W.K., Chang Y.E. & Lee P.G. (2006). Effects of incorporating spent bleaching clay from palm oil refining in the diets of African catfish, Clarias gariepinus. Asian Fisheries Science 19: 157-164.
  • Wang Y, Yuen K.H. & Ng W.K. (2006). Deposition of tocotrienols and tocopherols in the tissues of red hybrid tilapia, Oreochromis sp., fed a tocotrienol-rich fraction extracted from crude palm oil and its effect on lipid peroxidation. Aquaculture 253: 583-591.
  • Lim H.A., Bai S.C.C. & Ng W.K. (2005). Apparent nutrient digestibility and amino acid availability of several novel protein sources for tilapia, Oreochromis mossambicus. Journal of Aquaculture in the Tropics 20: 209-221.
  • Ng W.K. & Low S.Y. (2005). Evaluation of spent bleaching clay from palm oil refining as a feed ingredient in the diets of red hybrid tilapia, Oreochromis sp. Journal of Applied Aquaculture 17: 87-97.
  • Ng W.K. (2005). Palm fatty acid distillate supplementation in African catfish diets enhances growth and fillet quality. Global Aquaculture Advocate 9: 56-58.
  • Ng W.K. (2004). Researching the use of palm kernel cake in aquaculture feeds. Palm Oil Developments 41: 19-21. (Invited article).
  • Ng W.K. (2004). Palm oil as a novel dietary lipid source in aquaculture feeds. Palm Oil Developments 41: 14-18. (Invited article).
  • Ng W.K., Sigholt T. & Bell J.G. (2004). The influence of environmental temperature on the apparent nutrient and fatty acid digestibility in Atlantic salmon (Salmo salar L.) fed finishing diets containing different blends of fish oil, rapeseed oil and palm oil. Aquaculture Research 35: 1228-1237.
  • Ng W.K., Wang Y., Ketchimenin P. & Yuen K.H. (2004). Replacement of dietary fish oil with palm fatty acid distillate elevates tocopherol and tocotrienol concentrations and increases oxidative stability in the muscle of African catfish, Clarias gariepinus. Aquaculture 233: 423-437.
  • Tocher D.R., Fonseca-Madrigal J., Dick J.R., Ng W.K., Bell J.G. & Campbell P.J. (2004). Effects of water temperature and diets containing palm oil on fatty acid desaturation and oxidation in hepatocytes and intestinal enterocytes of rainbow trout (Oncorhynchus mykiss). Comparative Biochemistry and Physiology Part B 137: 49-63.
  • Ng W.K. & Chong C.Y. (2004). An overview of lipid nutrition with emphasis on alternative lipid sources in tilapia feeds. In: New Dimensions in Farmed Tilapia. Proceedings of the Sixth International Symposium on Tilapia in Aquaculture. (Bolivar R.B, Mair G.C. & Fitzsimmons K., editors), pp. 241-248. American Tilapia Association, USA.
  • Ng W.K. (2003). A comparison of two different methods in determining the protein requirement of the tropical bagrid catfish, Mystus nemurus. Journal of Aquaculture in the Tropics 18: 315-326.
  • Ng W.K., Campbell P.J., Dick J.R. & Bell J.G. (2003). Interactive effects of dietary palm oil concentration and water temperature on lipid digestibility in rainbow trout, Oncorhynchus mykiss. Lipids 38: 1031-1038.
  • Ng W.K., Lim P.K. & Boey P.L. (2003). Dietary lipid and palm oil source affects growth, fatty acid composition and muscle -tocopherol concentration of African catfish, Clarias gariepinus. Aquaculture 215: 229-243.
  • Ng W.K. (2002). Potential of palm oil utilisation in aquaculture feeds. Asia Pacific Journal of Clinical Nutrition 11: 473-476.
  • Ng W.K., Lim H.A., Lim S.L. & Ibrahim C.O. (2002). Nutritive value of palm kernel meal pretreated with enzyme or fermented with Trichoderma koningii (Oudemans) as a dietary ingredient for red hybrid tilapia (Oreochromis sp.). Aquaculture Research 33: 1199-1207.
  • Ng W.K. & Chen M.L. (2002). Replacement of soybean meal with palm kernel meal in practical diets for hybrid Asian-African catfish, Clarias macrocephalus x C. gariepinus. Journal of Applied Aquaculture 12: 67-76.
  • Ng W.K. & Chong K.K. (2002). The nutritive value of palm kernel meal and the effect of enzyme supplementation in practical diets for red hybrid tilapia. Asian Fisheries Science 15: 167-176.
  • Ng W.K. (2002). The nutrient requirements of clariid catfishes. Aquafeed International 5: 14-18.
  • Ng W.K., Lim P.K. & Sidek H. (2001). The influence of a dietary lipid source on growth, muscle fatty acid composition and erythrocyte osmotic fragility of hybrid tilapia. Fish Physiology and Biochemistry 25: 301-310.
  • Ng W.K., Ang L.P. & Liew F.L. (2001). An evaluation of mineral supplementation of fish meal-based diets for African catfish. Aquaculture International 9: 277-282.
  • Ng W.K., Liew F.L., Ang L.P. & Wong K.W. (2001). Potential of mealworm (Tenebrio molitor) as an alternative protein source in practical diets for African catfish, Clarias gariepinus. Aquaculture Research 32 (s1): 273-280.
  • Lim H.A., Ng W.K., Lim S.L. & Ibrahim C. O. (2001). Contamination of palm kernel meal with Aspergillus flavus affects its nutritive value in pelleted feed for tilapia, Oreochromis mossambicus. Aquaculture Research 32: 895-905.
  • Lim P.K., Boey P.L. & Ng W.K. (2001). Dietary palm oil level affects growth performance, protein retention and tissue vitamin E concentration of African catfish, Clarias gariepinus. Aquaculture 202: 101-112.
  • Ng W.K., Soon S.C. & Hashim R. (2001). The dietary protein requirement of a bagrid catfish, Mystus nemurus (Cuvier & Valenciennes), determined using semipurified diets of varying protein level. Aquaculture Nutrition 7: 45-52.
  • Lim H.A., Ng W.K., Hashim R. & Ali A. (2001). The nutritive value of winged bean and soybean meals in pelleted feed for a tropical bagrid catfish, Mystus nemurus. Journal of Bioscience 12: 123-132.
  • Ng W.K., Tee M.C. & Boey P.L. (2000). Evaluation of crude palm oil and refined palm olein as dietary lipids in pelleted feeds for a tropical bagrid catfish, Mystus nemurus (Cuvier & Valenciennes). Aquaculture Research 31: 337-348.
  • Ng W.K., Lu K.S., Hashim R. & Ali A. (2000). Effects of feeding rate on growth, feed utilization and body composition of a tropical bagrid catfish. Aquaculture International 8: 19-29.
  • Ng W.K., Keembiyehetty C.N. & Wilson R.P. (1998). Bioavailability of niacin from feed ingredients commonly used in feeds for channel catfish, Ictalurus punctatus. Aquaculture 161: 393-404.
  • Ng W.K. & Wilson R.P. (1997). Chromic oxide inclusion in the diet did not alter glucose utilization or tissue chromium retention by channel catfish, Ictalurus punctatus. Journal of Nutrition 127: 2357-2362.
  • Ng W.K., Serrini G., Zhang Z. & Wilson R.P. (1997). Niacin requirement and inability of tryptophan to act as a precursor of NAD+ in channel catfish, Ictalurus punctatus. Aquaculture 152: 273-285.
  • Ng W.K., Hung S.S.O. & Herold M.A. (1996). Poor utilization of dietary amino acids by white sturgeon. Fish Physiology and Biochemistry 15: 131-142.
  • Ng W.K. & Hung S.S.O. (1995). Estimating the ideal dietary indispensable amino acid pattern for growth of white sturgeon, Acipenser transmontanus (Richardson). Aquaculture Nutrition 1: 85-94.
  • Ng W.K. & Hung S.S.O. (1994). Amino acid composition of whole body, egg and selected tissues of white sturgeon, Acipenser transmontanus. Aquaculture 126: 329-339.
  • Ng W.K. & Wee K.L. (1989). The nutritive value of cassava leaf meal in pelleted feed for Nile tilapia. Aquaculture 83: 45-58.