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Mohd Razip Samian

 

Professor
B.Sc.Tech. (Hons), University of Wales Institute of
Science and Technology, NZ
M.Sc.(Biotech.), Ph.D., University of New South
Wales, Aust.
Ext. 4007: razip@macmail.com
Biotechnology

Research Profile

The research in my laboratory centres around 2 sub-disciplines: 1) molecular genetics of bioplastic (polyhydroxyalkanoic acid, PHA) production in bacteria and 2) structural biology of proteins.

 

Together with colleagues in the school, my lab focusses on using molecular genetics to modify the workhorse for PHA production, Cupriavidus necator H16, to convert plant oils into designer bioplastics. C. necator is able to convert plant oils into PHA efficiently. Figures up to 95% cell dry weight has been reported. The PHA produced naturally, however, possess unfavourable properties such as brittleness and lack of ductility. Designer PHA can be produced to have a range of properties suitable to particular applications. The second sub-discipline is on structural biology, specifically on the the PHA synthase enzyme – the polymerase responsible for synthesising the PHA polyester. Several works had been completed to identify the catalytic triad and key amino acids that are involved in the dimerisation of the PHA synthase. Currently we are working on computer simulation of the PHA synthase as well as trying to determine the 3-D structure by X-ray crystallography. 


Current Research

Collaborators

  • RIKEN, Japan

Selected Publications

  • Tan Y, Neo P-C, Najimudin N, Sudesh K, Tengku Muhammad TS, Othman, AS and Samian R (2010) Cloning and characterization of poly(3-hydroxybutyrate) biosynthesis genes from Pseudomonas sp. USM 4-55. J Basic Microbiol. 50(2):179-189
  • Chee, J. Y, Tan, Y., Samian, R., and Sudesh K. (2010) Isolation and Characterization of a Burkholderia sp. USM (JCM15050) Capable of Producing Polyhydroxyalkanoate (PHA) from Triglycerides, Fatty Acids and Glycerols. Journal of Polymers and the Environment. DOI: 10.1007/s10924-010-0204-1
  • Zahraa A Jarjes, Mohammed Razip Samian, Sulaiman Ab Ghani (2011) Electrochemical Study of Glycerol of Cooking Palm Oil with Glycerol Dehydrogenase at Polymers Modified Electrodes. Int. J. Electrochem. Sci., 6:1317-1330
  • EBB Ong, TS Tengku Muhammad, MR Samian and N Najimudin (2011) Purification of the Bacillus subtilis regulatory protein AlsR an the localization of its binding site. Asia Pac. J. Mol. Biol. Biotechnol. 19:29-37
  • Jarjes, Z. A., Samian, M. R. and Ghani, A. A. B (2011) Electro-oxidation of glycerol from cooking palm oil by dehydrogenanse enzymes immobilized on poly(o-phenylenediamine) modified electrodes. Journal of Materials Science and Engineering B 1 603-610
  • Blessia, T. F., Sharmila, D. J. S., Samian, M. R., Arsad, H. And Jamil, N. F. (2012) Structural analysis and molecular dynamics study of PHB synthase. International Research Journal of Pharmacy. 3(2):251-256
  • Jarjes, Z. A., Samian, M. R. And Ghani, S. A. (2012) Bio-electrode in mechanistic study of lipoxygenase with fatty acids from cooking palm oil. The Open Electrochemistry Journal 4:13-19
  • Yam H, Abdul Rahim A, Gim Luan O, Samian R, Abdul Manaf U, Mohamad S, Najimudin N. Validation of a Burkholderia pseudomallei hypothetical protein and determination of its translational start codon using chromosomal integration of His-Tag coding sequence. Protein J. 2012 Mar;31(3):246-9. doi: 10.1007/s10930-012-9398-5
  • Habibi, N., Hashim, S. Z. M. Rodrigues, C. A. And Samian, M. R. (2013) A review of CADs, languages and data models for synthetic biology. Jurnal Teknologi. 63(1):87-96 doi: 10.11113/jt.v63.1644
  • Habibi, N. Samian, R. And Hashim, S. Z. M. 2013 EcoliOverExpressionDB: A database of recombinant protein overexpression in E. Coli. Protein Expression and Purification. 95:92-95. http://dx.doi.org/10.1016/j.pep.2013.11.014
  • Habibi N., Hashim S. Z. M., Alireza N. and Samian M. R. 2014. A review of machine learning methods to predict the solubility of overexpressed recombinant proteins in Escherichia coli. BMC Bioinformatics 15:134 doi:10.1186/1471-2105-15-134

 

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