B.Sc.(Hons.) Eng., University of Guelph, Canada.
M.A.Sc., Ph.D., University of Waterloo, Canada
Ext. 6163; email@example.com
Human proteins are increasingly used today in pharmaceutical applications rather than extracts from animals or human plasma due to the lower immune response and reduced risk of transmission of diseases. The selection of a host for the production of a biological molecule has a substantial effect on the isolation and purifi cation protocols employed, along with their respective cost implications.
The use of microorganisms in producing metabolic products of commercial significance, particularly human proteins, has often been problematic.
Problems include low growth rates, formation of contaminating proteins and secretion of large amount of proteases which degrade the target product.
Bioprocessing strategies may be used to alleviate these problems.
- Production of human growth hormone in Pichia pastoris fermentation and downstream processing.
- Fermentation of recombinant Bacillus megaterium expressing human growth hormone.
- Kinetic studies on the production of biodegradable plastic in Pseudomonas putida.
- Soon-Il Y., Ahmad Yahya, M. Malten, D. Cossar, W.A. Anderson, J.M. Scharer and M. Moo-Young. 2001. Peptidases affecting recombinant protein production by Streptomyces lividans. Can. J. Microbiol. 47:1137–1140.
- Soon-Il Yun, Ahmad Yahya, D. Cossar, W. A. Anderson, J. M., Scharer and M.Moo-Young. 2001. Temperature downshift increases recombinant cytokine titer in Streptomyces lividans fermentation. Biotech. Lett. 23: 1903-1905.
- Ahmad R.M. Yahya, W. A. Anderson and M. Moo-Young. 1998. Ester synthesis in lipasecatalyzed reactions. Enz. Microb. Technol. 23: 438-450.
- Ahmad R.M. Yahya and Lillian Liao (eds.) 2001. Introductory Biotechnology, a laboratory manual for Introductory Biotechnology course at the Univ. of Waterloo.